General information
PDB ID 5NSR
Title CRYO-EM STRUCTURE OF RNA POLYMERASE-SIGMA54 HOLO ENZYME WITH PROMOTER DNA CLOSED COMPLEX
PDB header TRANSCRIPTION
Date 2017-04-26
Experimental method ELECTRON MICROSCOPY
Resolution (A) 3.8
Kind dna
Organism ESCHERICHIA COLI K-12, KLEBSIELLA PNEUMONIAE
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PDB PDBe MMDB Jena OCA
CATH PDBsum HSSP PDBePISA UniProt
ProteopediA BIPA
Composition of PDB entry and biounits
File: Protein chains: DNA chains: RNA chains: No. of models: Action:
PDB entry:
pdb5nsr.pdb ABCDEM FG 0
Biounits:
5nsr.pdb1.pdb ABCDEM FG 1
Pfam domains
Name: Type: Domain: E-value: Significance: Clan: Prot. chain: Start res.: End res.: Nuc. chains: Int. mode: Action:
RNA_pol_A_bac Domain P0A7Z4_58-186 9.1e-24 1 No_clan A 58 185   
RNA_pol_L Domain P0A7Z4_29-228 1.2e-22 1 CL0509 A 29 228   
RNA_pol_A_bac Domain P0A7Z4_58-186 7.8e-24 1 No_clan B 58 186   
RNA_pol_L Domain P0A7Z4_29-228 1.1e-22 1 CL0509 B 29 228   
RNA_pol_Rpb2_6 Domain P0A8V2_717-1264 3.3e-160 1 CL0410 C 717 1264   
RNA_pol_Rpb2_3 Domain P0A8V2_513-581 1.7e-29 1 No_clan C 513 581   
RNA_pol_Rpb2_45 Domain P0A8V2_591-656 2.1e-27 1 No_clan C 591 656   
RNA_pol_Rpb2_7 Domain P0A8V2_1266-1342 4.5e-27 1 No_clan C 1266 1341   
RNA_pol_Rpb2_2 Domain P0A8V2_329-454 6.1e-15 1 No_clan C 329 454   
RNA_pol_Rpb2_2 Domain P0A8V2_151-276 2.7e-07 1 No_clan C 151 276   
RNA_pol_Rpb1_1 Domain P0A8T7_15-342 8e-85 1 No_clan D 15 342   
RNA_pol_Rpb1_5 Domain P0A8T7_766-1317 1.8e-75 1 No_clan D 766 1317   
RNA_pol_Rpb1_2 Domain P0A8T7_386-486 4.1e-27 1 No_clan D 386 486   
RNA_pol_Rpb1_3 Domain P0A8T7_489-644 4.9e-27 1 No_clan D 489 644   
RNA_pol_Rpb1_4 Domain P0A8T7_673-764 1.4e-18 1 No_clan D 673 764   
RNA_pol_Rpb1_2 Domain P0A8T7_344-401 1.3e-07 1 No_clan D 344 401   
Sigma54_DBD Domain P06223_316-473 3.5e-57 1 CL0123 M 316 473 F   
Sigma54_CBD Domain P06223_118-257 2.8e-40 1 CL0123 M 43 262   
RNA_pol_Rpb2_1 Family P0A8V2_26-500 2.6e-58 1 No_clan C 26 500   
RNA_pol_Rpb6 Family P0A800_8-60 3.4e-17 1 No_clan E 8 60   
SCOP domains
No SCOP domains
GO terms
Protein chains: GO type: GO description:
A/B=1-329 C cytoplasm
A/B=1-329 C cytosol
A/B=1-329 C membrane
A/B=1-329 F DNA binding
A/B=1-329 F DNA-directed 5'-3' RNA polymerase activity
A/B=1-329 F protein dimerization activity
A/B=1-329 P transcription, DNA-templated
C=1-1342 C cytoplasm
C=1-1342 C cytosol
C=1-1342 C membrane
C=1-1342 F DNA binding
C=1-1342 F DNA-directed 5'-3' RNA polymerase activity
C=1-1342 F ribonucleoside binding
C=1-1342 P response to antibiotic
C=1-1342 P transcription, DNA-templated
D=1-1407 C cytoplasm
D=1-1407 C cytosol
D=1-1407 C membrane
D=1-1407 F DNA binding
D=1-1407 F DNA-directed 5'-3' RNA polymerase activity
D=1-1407 F magnesium ion binding
D=1-1407 F zinc ion binding
D=1-1407 P response to antibiotic
D=1-1407 P transcription, DNA-templated
E=1-91 C cytosol
E=1-91 C RNA polymerase complex
E=1-91 F DNA binding
E=1-91 F DNA-directed 5'-3' RNA polymerase activity
E=1-91 P protein-containing complex assembly
E=1-91 P transcription, DNA-templated
M=1-477 F DNA binding
M=1-477 F DNA-binding transcription activator activity
M=1-477 F DNA-directed 5'-3' RNA polymerase activity
M=1-477 F sigma factor activity
M=1-477 P DNA-templated transcription, initiation
M=1-477 P nitrogen fixation
Sequences
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5nsr.pdb1.pdb:   [ download sequences in FASTA format ]
F (dna): 
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G (dna): 
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A (protein): 
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B (protein): 
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C (protein): 
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D (protein): 
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E (protein): 
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M (protein): 
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